Coding

Part:BBa_K5374008:Design

Designed by: James Wang   Group: iGEM24_TestDaily-I-Beijing   (2024-09-19)


BMP-4 (Bone Morphogenetic Protein-4).BMP-4 is a signaling protein that plays a key role in bone form


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 61
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 184
    Illegal PstI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The recombinant BMP-4 sequence was codon-optimized for expression in E. coli. Additionally, its structure was carefully maintained to preserve the protein’s bioactivity in osteogenic pathways.


Source

BMP-4 is derived from human genes but can be produced recombinantly using E. coli or other systems.

References