Coding
Part:BBa_K5374008:Design
Designed by: James Wang Group: iGEM24_TestDaily-I-Beijing (2024-09-19)
BMP-4 (Bone Morphogenetic Protein-4).BMP-4 is a signaling protein that plays a key role in bone form
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 184
Illegal PstI site found at 265 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 61
Illegal PstI site found at 184
Illegal PstI site found at 265 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 184
Illegal PstI site found at 265 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 184
Illegal PstI site found at 265 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The recombinant BMP-4 sequence was codon-optimized for expression in E. coli. Additionally, its structure was carefully maintained to preserve the protein’s bioactivity in osteogenic pathways.
Source
BMP-4 is derived from human genes but can be produced recombinantly using E. coli or other systems.